Effect of Fetal Bovine Serum Concentration towards Vero Cells Growth on Culture in DMEM Medium

Cell culture requires a suitable medium and environment for in vivo conditions. Conditions are created by regulating temperature, pH, oxygen, CO2, osmotic pressure, surfaces to adhere cells, nutrients, vitamins, protection against toxic substances, hormones, growth factors that regulate cell differentiation. In general, good occurs at pH of 7.0 - 7.6. Fetal Bovine Serum (FBS) is one the supplement media support growth. The purpose this study was determine effect using FBS supplementation with various concentrations on vero DMEM (Dulbecco's modified eagle medium) media. Vero has been successfully carried out observing its 14 days. process begins manufacture consisting FBS. density each well 5% 10% concentration 1017 cells/well. By varying medium, difference number cells produced can be observed under microscope. results showed from higher than concentration. Thus, proves more it supports process.